Description
Description: This assay employs a two-site sandwich ELISA to quantitate ZNF655 in samples. An antibody specific for ZNF655 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZNF655 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF655 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF655 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: ZNF655 encodes a zinc finger protein.Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.VIK-1, did not contain such motifs but presented six tandemly arranged zinc-fingers and consensus Kruppel H-C links. VIK-1 interacted both with Vav-1 and cyclin-dependent kinase 4 through two independent domains and corresponded to a Vav C-Src homology domain (SH)3 partner able to shuttle between the nucleus and the cytoplasm exhibiting functional nuclear addressing and export sequences. The results indicated a restricted expression of the protein during the G1 phase and its overexpression resulted in an inhibition of the cell-cycle progression that was reversed in the presence of Vav 1